A rapid, simple, selective and precise UV-visible spectrophotometric method has been used for the estimation of caffeine content in various nine brands of tea powder consumed in Nepal. Numbers of reports are available for the estimation of caffeine in both official and published research papers. In Nepal, majority of the people consumed tea rather than coffee. Their daily life starts with a cup of tea. During our study we have found that no any specific research has been done about this chemical constituent on various brands of tea available in the market of Nepal. The amount of caffeine in tea brands were estimated by comparing the absorbance with reference caffeine using multiple standard method. The linearity range was found to be 2-12 μg/ml with a correlation coefficient of 0.998. From our study, the highest amount of caffeine content was found in Nepalese tea, Tokla (6.6212 μg/ml) as compared to other brands of tea such as Indian tea, Taj mahal (5.3939 μg/ml), Red label (5.4393 μg/ml) and Tata 5.8181(μg/ml) and Chinese tea Jasmine (5.4090 μg/ml). Thin layer chromatography was performed to ensure the separation of the spots. Our study may be the first in such setting in Nepal to assess this chemical constituent in different brands of tea.
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